Different Methods for DNA Purification

DNA purification is among the most commonly used and crucial procedures in molecular biology. The aim of DNA purification is the separation of the desired genetic material from the contaminant (proteins, cell membranes, and RNA). This is a critical procedure in nearly all molecular applications. It must be done correctly in order to obtain top-quality, usable DNA.

There are a variety of options for DNA purification. The choice is based on a variety of factors like the source materials, downstream applications, cost, and time constraints. Typical genomic and plasmid DNA purification methods include chemical treatment, enzymatic digestion, or mechanical destruction of cell samples or tissue followed by salting-out of the proteins and the precipitation of DNA with ethanol.

Ethanol precipitation is a https://mpsciences.com/2021/04/15/gene-synthesis-and-transcription-processes/ straightforward, inexpensive and quick method for desalting and concentration of DNA. DNA molecules accumulate in the presence of monovalent cations, such as sodium and then they are removed from the solution by using high concentrations of ethanol. This method is used to remove salts, organic compounds and other impurities. It is often used with other purification methods.

Anion exchange chromatography is yet another well-known method for DNA purification. The interaction between negatively charged DNA phosphate backbones and the positively charged surface molecules of resins bonds DNA in a solvent to positively-charged resins. During the binding and washing steps, contaminating molecules are removed from DNA through rigorous wash steps and the DNA that has been purified is eluted in low salt conditions.